Comprehensive chromatin proteomics resolves functional phases of pluripotency and identifies changes in regulatory components.

ABSTRACT: The establishment of cellular identity is driven by transcriptional and epigenetic regulators of the chromatin proteome - the chromatome. Comprehensive analyses of the chromatome composition and dynamics can therefore greatly improve our understanding of gene regulatory mechanisms. Here, we developed an accurate mass spectrometry (MS)-based proteomic method called Chromatin Aggregation Capture (ChAC) followed by Data-Independent Acquisition (DIA) and analyzed chromatome reorganizations during major phases of pluripotency. This enabled us to generate a comprehensive atlas of proteomes, chromatomes, and chromatin affinities for the ground, formative and primed pluripotency states, and to pinpoint the specific binding and rearrangement of regulatory components. These comprehensive datasets co

SUBMITTER: Ugur E 

PROVIDER: S-EPMC10085704 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature