PMI estimation through metabolomics and potassium analysis on animal vitreous humour.

ABSTRACT:

Introduction

The estimation of post-mortem interval (PMI) remains a major challenge in forensic science. Most of the proposed approaches lack the reliability required to meet the rigorous forensic standards.

Objectives

We applied ¹H NMR metabolomics to estimate PMI on ovine vitreous humour comparing the results with the actual scientific gold standard, namely vitreous potassium concentrations.

Methods

Vitreous humour samples were collected in a time frame ranging from 6 to 86 h after death. Experiments were performed by using ¹H NMR metabolomics and ion capillary analysis. Data were submitted to multivariate statistical data analysis.

Results

A multivariate calibration model was built to estimate PMI based on 47 vitreous humour samples. Th

SUBMITTER: Locci E 

PROVIDER: S-EPMC10085955 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature