Function and dynamics of the intrinsically disordered carboxyl terminus of β2 adrenergic receptor.

ABSTRACT: Advances in structural biology have provided important mechanistic insights into signaling by the transmembrane core of G-protein coupled receptors (GPCRs); however, much less is known about intrinsically disordered regions such as the carboxyl terminus (CT), which is highly flexible and not visible in GPCR structures. The β₂ adrenergic receptor's (β₂AR) 71 amino acid CT is a substrate for GPCR kinases and binds β-arrestins to regulate signaling. Here we show that the β₂AR CT directly inhibits basal and agonist-stimulated signaling in cell lines lacking β-arrestins. Combining single-molecule fluorescence resonance energy transfer (FRET), NMR spectroscopy, and molecular dynamics simulations, we reveal that the negatively charged β₂AR-CT serves as

SUBMITTER: Heng J 

PROVIDER: S-EPMC10085991 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature