Rapid and sensitive on-site genetic diagnostics of pest fruit flies using CRISPR-Cas12a.

ABSTRACT:

Background

Bactrocera zonata, a major fruit pest species, is gradually spreading west from its native habitat in East Asia. In recent years it has become a significant threat to the Mediterranean area, with the potential of invading Europe, the Americas, and Australia. To prevent it spreading, monitoring efforts in cultivation sites and border controls are carried out. Despite these efforts, and due to morphological similarities between B. zonata and other pests in relevant developmental stages, the monitoring process is challenging, time-consuming, and requires external assistance from professional laboratories. CRISPR-Cas12a genetic diagnostics has been rapidly developing in recent years and provides an efficient tool for the genetic identification of pathogens, viruses, and othe

SUBMITTER: Alon DM 

PROVIDER: S-EPMC10086973 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature