Molecular characterization of the immediate wound response of the solitary ascidian Polycarpa mytiligera.

ABSTRACT:

Background

Injury response is key to successful regeneration. Yet, transcriptome analyses of injury response were performed only on a handful of regenerative organisms. Here, we studied the injury response of the solitary ascidian Polycarpa mytiligera, an emerging model system, capable of regenerating any body part. We used the siphon as a model for studying transcriptional changes following injury, and identified genes that were activated in the initial 24 hours post amputation (hpa).

Results

Highly conserved genes, such as bone morphogenetic protein-1 (BMP1), growth hormone secretagogue receptor (GHSR) and IL-17, were upregulated by 12 hpa, yet their expression was sustained only in non-regenerating tissue fragments. We optimized fluorescent in situ hybridization, and foun

SUBMITTER: Hendin N 

PROVIDER: S-EPMC10087333 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature