OMIP-085: Cattle B-cell phenotyping by an 8-color panel.

ABSTRACT: This 8-color panel has been optimized to distinguish between functionally distinct subsets of cattle B cells in both fresh and cryopreserved peripheral blood mononuclear cells (PBMCs). Existing characterized antibodies against cell surface molecules (immunoglobulin light chain (S-Ig[L]), CD20, CD21, CD40, CD71, and CD138) enabled the discrimination of 24 unique populations within the B-cell population. This allows the identification of five putative functionally distinct B-cell subsets critical to infection and vaccination responses: (1) naïve B cells (B_Naïve ), (2) regulatory B cells (B_Reg ), (3) memory B cells (B_Mem ), (4) plasmablasts (PB), and (5) plasma cells (PC). Although CD3 and CD8α can be included as an additional dump channel, it does not signifi

SUBMITTER: Roos EO 

PROVIDER: S-EPMC10087846 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature