Software-Assisted Data Processing Workflow for Intact Glycoprotein Mass Spectrometry.

ABSTRACT: Intact protein analysis by mass spectrometry is important for several applications such as assessing post-translational modifications and biotransformation. In particular, intact protein analysis allows the detection of proteoforms that are commonly missed by other approaches such as proteolytic digestion followed by bottom-up analysis. Two quantification methods are mainly used for intact protein data quantification, namely the extracted ion and deconvolution approaches. However, a consensus with regard to a single best practice for intact protein data processing is lacking. Furthermore, many data processing tools are not fit-for-purpose and, as a result, the analysis of intact proteins is laborious and lacks the throughput required to be implemented for the analysis of clinical cohorts.

SUBMITTER: Moran AB 

PROVIDER: S-EPMC10088042 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature