Spatial Proteomics for Further Exploration of Missing Proteins: A Case Study of the Ovary.

ABSTRACT: In the quest for "missing proteins" (MPs), the proteins encoded by the human genome still lacking evidence of existence at the protein level, novel approaches are needed to detect this challenging group of proteins. The current count stands at 1,343 MPs, and it is likely that many of these proteins are expressed at low levels, in rare cell or tissue types, or the cells in which they are expressed may only represent a small minority of the tissue. Here, we used an integrated omics approach to identify and explore MPs in human ovaries. By taking advantage of publicly available transcriptomics and antibody-based proteomics data in the Human Protein Atlas (HPA), we selected 18 candidates for further immunohistochemical analysis using an exclusive collection of ovarian tissues from women and pa

SUBMITTER: Mear L 

PROVIDER: S-EPMC10088045 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature