Diagnostic TR-FRET assays for detection of antibodies in patient samples.

ABSTRACT: Serological assays are important diagnostic tools for surveying exposure to the pathogen, monitoring immune response post vaccination, and managing spread of the infectious agent among the population. Current serological laboratory assays are often limited because they require the use of specialized laboratory technology and/or work with a limited number of sample types. Here, we evaluate an alternative by developing time-resolved Förster resonance energy transfer (TR-FRET) homogeneous assays that exhibited exceptional versatility, scalability, and sensitivity and outperformed or matched currently used strategies in terms of sensitivity, specificity, and precision. We validated the performance of the assays measuring total immunoglobulin G (IgG) levels; antibodies against severe acute resp

SUBMITTER: Yue H 

PROVIDER: S-EPMC10088089 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature