METTL3 enhances dentinogenesis differentiation of dental pulp stem cells via increasing GDF6 and STC1 mRNA stability.

ABSTRACT:

Background

The dentinogenesis differentiation of dental pulp stem cells (DPSCs) is controlled by the spatio-temporal expression of differentiation related genes. RNA N6-methyladenosine (m⁶A) methylation, one of the most abundant internal epigenetic modification in mRNA, influences various events in RNA processing, stem cell pluripotency and differentiation. Methyltransferase like 3 (METTL3), one of the essential regulators, involves in the process of dentin formation and root development, while mechanism of METTL3-mediated RNA m⁶A methylation in DPSC dentinogenesis differentiation is still unclear.

Methods

Immunofluorescence staining and MeRIP-seq were performed to establish m⁶A modification profile in dentinogenesis differentiation. Lentivir

SUBMITTER: Pan Y 

PROVIDER: S-EPMC10088233 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature