Transcriptome analysis highlights the role of ferroptosis in palmitic acid-induced endothelial dysfunction.

ABSTRACT:

Background

Palmitic acid (PA) has a lipotoxic effect on blood vessels, leading to endothelial dysfunction and cell death. The underlying mechanisms are not yet fully understood.

Aim

We sought to investigate the effects of PA on endothelial cells, with an emphasis on ferroptosis.

Methods

Rat corpus cavernosum endothelial cells (RCCECs) and human umbilical vein endothelial cells (HUVECs) were treated with PA to induce a pattern of cell death, as evidenced by the evaluation of cell viability. The differentially expressed genes were measured via RNA sequencing to reveal potential mechanisms. The intracellular levels of glutathione (GSH), malondialdehyde (MDA), ferrous ion (Fe²⁺), and reactive oxygen species (ROS) were evaluated using commercial kits. Western bl

SUBMITTER: Tan XH 

PROVIDER: S-EPMC10089063 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature