Increased flexibility of the SARS-CoV-2 RNA-binding site causes resistance to remdesivir.

ABSTRACT: Mutations continue to accumulate within the SARS-CoV-2 genome, and the ongoing epidemic has shown no signs of ending. It is critical to predict problematic mutations that may arise in clinical environments and assess their properties in advance to quickly implement countermeasures against future variant infections. In this study, we identified mutations resistant to remdesivir, which is widely administered to SARS-CoV-2-infected patients, and discuss the cause of resistance. First, we simultaneously constructed eight recombinant viruses carrying the mutations detected in in vitro serial passages of SARS-CoV-2 in the presence of remdesivir. We confirmed that all the mutant viruses didn't gain the virus production efficiency without remdesivir treatment. Time course analyses of cellular viru

SUBMITTER: Torii S 

PROVIDER: S-EPMC10089321 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature