Enabling low-cost and robust essentiality studies with high-throughput transposon mutagenesis (HTTM).

ABSTRACT: Transposon-insertion sequencing (TIS) methods couple high density transposon mutagenesis with next-generation sequencing and are commonly used to identify essential or important genes in bacteria. However, this approach can be work-intensive and sometimes expensive depending on the selected protocol. The difficulty to process a high number of samples in parallel using standard TIS protocols often restricts the number of replicates that can be performed and limits the deployment of this technique to large-scale projects studying gene essentiality in various strains or growth conditions. Here, we report the development of a robust and inexpensive High-Throughput Transposon Mutagenesis (HTTM) protocol and validate the method using Escherichia coli strain BW25113, the parental strain of the KE

SUBMITTER: Champie A 

PROVIDER: S-EPMC10089323 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature