Cycloaddition enabled mutational profiling of 5-vinyluridine in RNA.

ABSTRACT: We report the detection of 5-vinyluridine (5-VUrd) in RNA at single nucleotide resolution via mutational profiling. Maleimide cycloadducts with 5-VUrd in RNA cause a stop in primer extension during reverse transcription, and the full-length cDNA product from reverse transcription contains misincorporation across the cycloadduct site.

SUBMITTER: Gupta M 

PROVIDER: S-EPMC10089805 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature