Nintedanib induces apoptosis in human pterygium cells through the FGFR2-ERK signalling pathway.

ABSTRACT:

Aim

To investigate whether nintedanib can inhibit pterygium cells through the fibroblast growth factor receptor 2 (FGFR2)/extracellular-signal-regulated kinase (ERK) pathway.

Methods

Human primary pterygium cells were cultured in vitro. After treatment with nintedanib, the cell morphology was observed under microscopy, the morphological changes of the nucleus were observed after DAPI staining, apoptosis was analyzed by Annexin-V FITC/PI double staining, and the changes of apoptosis-associated proteins were detected by Western blot. The binding ability of nintedanib to FGFR2 was predicted by molecular docking. Finally, by silencing FGFR2, we explored whether nintedanib inhibited FGFR2/ERK pathway.

Results

The results showed that nintedanib inhibited the growth o

SUBMITTER: Gong Y 

PROVIDER: S-EPMC10089899 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature