The lipoxygenase OsLOX10 affects seed longevity and resistance to saline-alkaline stress during rice seedlings.

ABSTRACT: Prolonged storage of rice seeds can lead to a decrease in seed vigor and seedling quality. The Lipoxygenase (LOX) gene family is widely distributed in plants, and LOX activity is closely related to seed viability and stress tolerance. In this study, the lipoxygenase OsLOX10 gene from the 9-lipoxygenase metabolic pathway was cloned from rice, and its roles in determining seed longevity and tolerance to saline-alkaline stress caused by Na₂CO₃ in rice seedlings were mainly investigated. CRISPR/Cas9 knockout of OsLOX10 increased seed longevity compared with the wild-type and OsLOX10 overexpression lines in response to artificial aging. The expression levels of other 9-lipoxygenase metabolic pathway related genes, such as LOX1, LOX2 and LOX3, were increased in the LOX10 ov

SUBMITTER: Wang F 

PROVIDER: S-EPMC10089987 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature