The use of the primary structure of the ITS1-ITS2 region for species identification in some submerged aquatic macrophytes of the genus Stuckenia.

ABSTRACT: Applicability of ITS1-ITS2 primary structure for species attribution of representatives of the genus Stuckenia was experimentally tested. Analysis of the ITS1-ITS2 region sequences of S. vaginata and S. pectinata from public databases showed that they differed by insertions/deletions and single or double nucleotide substitutions. Besides, the ITS1-ITS2 region of S. pectinata was shown to be represented by two haplotype groups designated as S. pectinata type A and S. pectinata type B with good bootstrap support in phylogenetic reconstructions. In 28 samples identified as S. pectinata, S. vaginata, S. macrocarpa and S. chakassiensis on the basis of morphology, the ITS1-ITS2 region was sequenced in this study. Three groups of samples with good bootstrap support were revealed to be correspondi

SUBMITTER: Mglinets AV 

PROVIDER: S-EPMC10090118 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature