Characterization, protein modeling, and molecular docking of factor C from Indonesian horseshoe crab (Tachypleus gigas).

ABSTRACT:

Background

Horseshoe crab (Tachypleus gigas) amebocytes are useful biomedical components for endotoxin detection, and their growing needs for biomedical purposes cause the horseshoe crab population to decline. Factor C synthesis via genetic engineering offers a solution to replace natural horseshoe crab's factor C and prevent its excessive harvest from nature. In response to these concerns, this study aimed to characterize the amebocyte lysates and factor C protein modeling of T. gigas originated from Banyuasin South Sumatra Estuary.

Methods and results

Sampling of T. gigas was carried out in Banyuasin South Sumatra Estuary, Indonesia. The endotoxin test or TAL (Tachypleus amebocyte lysates) assay was performed using gel coagulation method. Protein characterization of protea

SUBMITTER: Mustopa AZ 

PROVIDER: S-EPMC10090249 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature