Calponin 2 harnesses metabolic reprogramming to determine kidney fibrosis.

ABSTRACT:

Objective

In the fibrotic kidneys, the extent of a formed deleterious microenvironment is determined by cellular mechanical forces. This process requires metabolism for energy. However, how cellular mechanics and metabolism are connected remains unclear.

Methods

A multi-disciplinary approach was employed: the fibrotic kidney disease models were induced by renal ischemia-reperfusion injury and unilateral ureteral obstruction in Calponin 2 (CNN2) knockdown mice. Proteomics, bioinformatics, and in vivo and in vitro molecular experimental pathology studies were performed.

Result

Our proteomics revealed that actin filament binding and cell metabolism are the two most dysregulated events in the fibrotic kidneys. As a prominent actin stabilizer, CNN2 was predominantly expres

SUBMITTER: Gui Y 

PROVIDER: S-EPMC10090436 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature