AutoComet: A fully automated algorithm to quickly and accurately analyze comet assays.

ABSTRACT: DNA damage is a common cellular feature seen in cancer and neurodegenerative disease, but fast and accurate methods for quantifying DNA damage are lacking. Comet assays are a biochemical tool to measure DNA damage based on the migration of broken DNA strands towards a positive electrode, which creates a quantifiable 'tail' behind the cell. However, a major limitation of this approach is the time needed for analysis of comets in the images with available open-source algorithms. The requirement for manual curation and the laborious pre- and post-processing steps can take hours to days. To overcome these limitations, we developed AutoComet, a new open-source algorithm for comet analysis that utilizes automated comet segmentation and quantification of tail parameters. AutoComet first segments

SUBMITTER: Barbe L 

PROVIDER: S-EPMC10090439 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature