Phosphorylation-linked complex profiling identifies assemblies required for Hippo signal integration.

ABSTRACT: While several computational methods have been developed to predict the functional relevance of phosphorylation sites, experimental analysis of the interdependency between protein phosphorylation and Protein-Protein Interactions (PPIs) remains challenging. Here, we describe an experimental strategy to establish interdependencies between protein phosphorylation and complex formation. This strategy is based on three main steps: (i) systematically charting the phosphorylation landscape of a target protein; (ii) assigning distinct proteoforms of the target protein to different protein complexes by native complex separation (AP-BNPAGE) and protein correlation profiling; and (iii) analyzing proteoforms and complexes in cells lacking regulators of the target protein. We applied this strategy to YA

SUBMITTER: Uliana F 

PROVIDER: S-EPMC10090947 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature