Vibrio natriegens genome-scale modeling reveals insights into halophilic adaptations and resource allocation.

ABSTRACT: Vibrio natriegens is a Gram-negative bacterium with an exceptional growth rate that has the potential to become a standard biotechnological host for laboratory and industrial bioproduction. Despite this burgeoning interest, the current lack of organism-specific qualitative and quantitative computational tools has hampered the community's ability to rationally engineer this bacterium. In this study, we present the first genome-scale metabolic model (GSMM) of V. natriegens. The GSMM (iLC858) was developed using an automated draft assembly and extensive manual curation and was validated by comparing predicted yields, central metabolic fluxes, viable carbon substrates, and essential genes with empirical data. Mass spectrometry-based proteomics data confirmed the translation of at least 76% of

SUBMITTER: Coppens L 

PROVIDER: S-EPMC10090949 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature