Modeling the Orthosteric Binding Site of the G Protein-Coupled Odorant Receptor OR5K1.

ABSTRACT: With approximately 400 encoding genes in humans, odorant receptors (ORs) are the largest subfamily of class A G protein-coupled receptors (GPCRs). Despite its high relevance and representation, the odorant-GPCRome is structurally poorly characterized: no experimental structures are available, and the low sequence identity of ORs to experimentally solved GPCRs is a significant challenge for their modeling. Moreover, the receptive range of most ORs is unknown. The odorant receptor OR5K1 was recently and comprehensively characterized in terms of cognate agonists. Here, we report two additional agonists and functional data of the most potent compound on two mutants, L104^3.32 and L255^6.51. Experimental data was used to guide the investigation of the binding modes of OR5K1

SUBMITTER: Nicoli A 

PROVIDER: S-EPMC10091413 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature