Upregulation of BMI1-suppressor miRNAs (miR-200c, miR-203) during terminal differentiation of colon epithelial cells.

ABSTRACT:

Background

MicroRNAs (miRNAs) are key regulators of stem cell functions, including self-renewal and differentiation. In this study, we aimed to identify miRNAs that are upregulated during terminal differentiation in the human colon epithelium, and elucidate their role in the mechanistic control of stem cell properties.

Methods

"Bottom-of-the-crypt" (EPCAM⁺/CD44⁺/CD66a^low) and "top-of-the-crypt" (EPCAM⁺/CD44^neg/CD66a^high) epithelial cells from 8 primary colon specimens (6 human, 2 murine) were purified by flow cytometry and analyzed for differential expression of 335 miRNAs. The miRNAs displaying the highest upregulation in "top-of-the-crypt" (terminally differentiated) epithelial cells were tested for positive

SUBMITTER: Hisamori S 

PROVIDER: S-EPMC10091510 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature