New insights into posttranslational modifications of proteins during bull sperm capacitation.

ABSTRACT:

Background

Due to the unique nature of spermatozoa, which are transcriptionally and translationally silent, the regulation of capacitation is based on the formation of posttranslational modifications of proteins (PTMs). However, the interactions between different types of PTMs during the capacitation remain unclear. Therefore, we aimed to unravel the PTM-based regulation of sperm capacitation by considering the relationship between tyrosine phosphorylation and reversible oxidative PTMs (oxPTMs), i.e., S-nitrosylation and S-glutathionylation. Since reversible oxPTMs may be closely related to peroxyredoxin (PRDX) activity, the second aim was to verify the role of PRDXs in the PTM-based regulation of capacitation.

Methods

Cryopreserved bull sperm were capacitated in vitro with

SUBMITTER: Mostek-Majewska A 

PROVIDER: S-EPMC10091539 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature