Promoting the application of Pinus thunbergii Parl. to enhance the growth and survival rates of post-germination somatic plantlets.

ABSTRACT:

Objective

There is a growing need for nematode resistant Pinaceae species plantlets to cope with the global scale degradation of coniferous forests, due to the prevalence of pine wilt disease. One of the bottlenecks that limits the commercialization of Pinaceae species plantlets is regeneration following their transfer from controlled sterile environments to the field while maintaining high survival rates.

Methods

The growth factors of somatic plantlets (SPs), such as sucrose, media, culture substrate, brassinolide and spectrum were investigated to promote the application of somatic nematode-resistant P. thunbergii plants in afforestation.

Results

The 1/2 WPM liquid medium, culture substrate (perlite and vermiculite =1:1), and carbohydrate (20 g/L sucrose) were effect

SUBMITTER: Sun T 

PROVIDER: S-EPMC10091573 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature