Development of an antibody-ligand fusion protein scFvCD16A_-sc4-1BBL in Komagataella phaffii with stimulatory activity for Natural Killer cells.

ABSTRACT:

Background

Natural killer (NK) cell-based immunotherapies have demonstrated substantial potential for the treatment of hematologic malignancies. However, its application is limited due to the difficulty in the production of a large number of NK cells in vitro and the insufficient therapeutic efficacy against solid tumors in vivo. Engineered antibodies or fusion proteins targeting activating receptors and costimulatory molecules of NK cells have been developed to encounter these problems. They are mostly produced in mammalian cells with high cost and long processing times. Yeast systems, such as Komagataella phaffii, present a convenient manipulation of microbial systems with the key advantages of improved folding machinery and low cost.

Results

In this study, we designed an

SUBMITTER: Li Y 

PROVIDER: S-EPMC10091686 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature