Surface acoustic wave microfluidics for repetitive and reversible temporary immobilization of C. elegans.

ABSTRACT: Caenorhabditis elegans is an important genetic model for neuroscience studies, used for analyses of how genes control connectivity, neuronal function, and behavior. To date, however, most studies of neuronal function in C. elegans are incapable of obtaining microscopy imaging with subcellular resolution and behavior analysis in the same set of animals. This constraint stems from the immobilization requirement for high-resolution imaging that is incompatible with behavioral analysis using conventional immobilization techniques. Here, we present a novel microfluidic device that uses surface acoustic waves (SAW) as a non-contact method to temporarily immobilize worms for a short period (30 seconds). We optimize the SAW based protocol for rapid switching between free-swimming and

SUBMITTER: Sridhar N 

PROVIDER: S-EPMC10091851 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature