Marked oestrous cycle-dependent regulation of rat arterial K_V 7.4 channels driven by GPER1.

ABSTRACT:

Background and purpose

Kcnq-encoded K_V 7 channels (termed K_V 7.1-5) regulate vascular smooth muscle cell (VSMC) contractility at rest and as targets of receptor-mediated responses. However, the current data are mostly derived from males. Considering the known effects of sex, the oestrous cycle and sex hormones on vascular reactivity, here we have characterised the molecular and functional properties of K_V 7 channels from renal and mesenteric arteries from female Wistar rats separated into di-oestrus and met-oestrus (F-D/M) and pro-oestrus and oestrus (F-P/E).

Experimental approach

RT-qPCR, immunocytochemistry, proximity ligation assay and wire myography were performed in renal and mesenteric arteries. Circulating sex hormone concentrations we

SUBMITTER: Baldwin SN 

PROVIDER: S-EPMC10091994 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature