Specific Lipid Studies in Complex Membranes by Solid-State NMR Spectroscopy.

ABSTRACT: Specific interactions with phospholipids are often critical for the function of proteins or drugs, but studying these interactions at high resolution remains difficult, especially in complex membranes that mimic biological conditions. In principle, molecular interactions with phospholipids could be directly probed by solid-state NMR (ssNMR). However, due to the challenge to detect specific lipids in mixed liposomes and limited spectral sensitivity, ssNMR studies of specific lipids in complex membranes are scarce. Here, by using purified biological ¹³ C,¹⁵ N-labeled phospholipids, we show that we can selectively detect traces of specific lipids in complex membranes. In combination with ¹ H-detected ssNMR, we show that our approach provides unprecedented high

SUBMITTER: van Beekveld RAM 

PROVIDER: S-EPMC10092488 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature