High-Throughput Microscopy Analysis of Mitochondrial Membrane Potential in 2D and 3D Models.

ABSTRACT: Recent proteomic, metabolomic, and transcriptomic studies have highlighted a connection between changes in mitochondria physiology and cellular pathophysiological mechanisms. Secondary assays to assess the function of these organelles appear fundamental to validate these -omics findings. Although mitochondrial membrane potential is widely recognized as an indicator of mitochondrial activity, high-content imaging-based approaches coupled to multiparametric to measure it have not been established yet. In this paper, we describe a methodology for the unbiased high-throughput quantification of mitochondrial membrane potential in vitro, which is suitable for 2D to 3D models. We successfully used our method to analyze mitochondrial membrane potential in monolayers of human fibroblasts, neural st

SUBMITTER: Vianello C 

PROVIDER: S-EPMC10093082 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature