Isolation and Characterization of Novel Canine Osteosarcoma Cell Lines from Chemotherapy-Naive Patients.

ABSTRACT: The present study aimed to establish novel canine osteosarcoma cell lines (COS3600, COS3600B, COS4074) and characterize the recently described COS4288 cells. The established D-17 cell line served as a reference. Analyzed cell lines differed notably in their biological characteristics. Calculated doubling times were between 22 h for COS3600B and 426 h for COS4074 cells. COS3600B and COS4288 cells produced visible colonies after anchorage-independent growth in soft agar. COS4288 cells were identified as cells with the highest migratory capacity. All cells displayed the ability to invade through an artificial basement membrane matrix. Immunohistochemical analyses revealed the mesenchymal origin of all COS cell lines as well as positive staining for the osteosarcoma-relevant proteins alkaline

SUBMITTER: Leitner N 

PROVIDER: S-EPMC10093184 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature