Raman Spectroscopic Imaging of Human Bladder Resectates towards Intraoperative Cancer Assessment.

ABSTRACT: Raman spectroscopy offers label-free assessment of bladder tissue for in vivo and ex vivo intraoperative applications. In a retrospective study, control and cancer specimens were prepared from ten human bladder resectates. Raman microspectroscopic images were collected from whole tissue samples in a closed chamber at 785 nm laser excitation using a 20× objective lens and 250 µm step size. Without further preprocessing, Raman images were decomposed by the hyperspectral unmixing algorithm vertex component analysis into endmember spectra and their abundancies. Hierarchical cluster analysis distinguished endmember Raman spectra that were assigned to normal bladder, bladder cancer, necrosis, epithelium and lipid inclusions. Interestingly, Raman spectra of microplastic particles, pigments or car

SUBMITTER: Krafft C 

PROVIDER: S-EPMC10093366 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature