Macrophages Promote Tumor Cell Extravasation across an Endothelial Barrier through Thin Membranous Connections.

ABSTRACT: Macrophages are important players involved in the progression of breast cancer, including in seeding the metastatic niche. However, the mechanism by which macrophages in the lung parenchyma interact with tumor cells in the vasculature to promote tumor cell extravasation at metastatic sites is not clear. To mimic macrophage-driven tumor cell extravasation, we used an in vitro assay (eTEM) in which an endothelial monolayer and a matrigel-coated filter separated tumor cells and macrophages from each other. The presence of macrophages promoted tumor cell extravasation, while macrophage conditioned media was insufficient to stimulate tumor cell extravasation in vitro. This finding is consistent with a requirement for direct contact between macrophages and tumor cells. We observed the presence o

SUBMITTER: Genna A 

PROVIDER: S-EPMC10093384 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature