Evaluation of Alternative Colony Hybridization Methods for Pathogenic Vibrios.

ABSTRACT: Vibrios, such as Vibrio parahaemolyticus, are naturally occurring halophilic bacteria that are a major cause of foodborne illness. Because of their autochthonous nature, managing vibrio levels in marine and estuarine environments is impossible. Instead, it is crucial to reliably enumerate their abundance to minimize human exposure. One method of achieving this is the direct plating/colony hybridization (DP/CH) method, which has been used to efficiently quantify pathogenic vibrios in oysters and other seafood products. Although successful, the method relies on proprietary resources. We examined alternative approaches, assessed the influence of the reagent suppliers' source on enumeration accuracy, and made experimental adjustments that maximized efficiency, sensitivity, and specifici

SUBMITTER: Schwartz AM 

PROVIDER: S-EPMC10093671 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature