Accurate Prediction of Transcriptional Activity of Single Missense Variants in HIV Tat with Deep Learning.

ABSTRACT: Tat is an essential gene for increasing the transcription of all HIV genes, and affects HIV replication, HIV exit from latency, and AIDS progression. The Tat gene frequently mutates in vivo and produces variants with diverse activities, contributing to HIV viral heterogeneity as well as drug-resistant clones. Thus, identifying the transcriptional activities of Tat variants will help to better understand AIDS pathology and treatment. We recently reported the missense mutation landscape of all single amino acid Tat variants. In these experiments, a fraction of double missense alleles exhibited intragenic epistasis. However, it is too time-consuming and costly to determine the effect of the variants for all double mutant alleles through experiments. Therefore, we propose a combined GigaAssay/

SUBMITTER: Derbel H 

PROVIDER: S-EPMC10093788 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature