Innovative Computerized Dystrophin Quantification Method Based on Spectral Confocal Microscopy.

ABSTRACT: Several clinical trials are working on drug development for Duchenne and Becker muscular dystrophy (DMD and BMD) treatment, and, since the expected increase in dystrophin is relatively subtle, high-sensitivity quantification methods are necessary. There is also a need to quantify dystrophin to reach a definitive diagnosis in individuals with mild BMD, and in female carriers. We developed a method for the quantification of dystrophin in DMD and BMD patients using spectral confocal microscopy. It offers the possibility to capture the whole emission spectrum for any antibody, ensuring the selection of the emission peak and allowing the detection of fluorescent emissions of very low intensities. Fluorescence was evaluated first on manually selected regions of interest (ROIs), proving the usefu

SUBMITTER: Codina A 

PROVIDER: S-EPMC10094132 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature