Uptake of Tropheryma whipplei by Intestinal Epithelia.

ABSTRACT:

Background

Tropheryma whipplei (TW) can cause different pathologies, e.g., Whipple's disease and transient gastroenteritis. The mechanism by which the bacteria pass the intestinal epithelial barrier, and the mechanism of TW-induced gastroenteritis are currently unknown.

Methods

Using ex vivo disease models comprising human duodenal mucosa exposed to TW in Ussing chambers, various intestinal epithelial cell (IEC) cultures exposed to TW and a macrophage/IEC coculture model served to characterize endocytic uptake mechanisms and barrier function.

Results

TW exposed ex vivo to human small intestinal mucosae is capable of autonomously entering IECs, thereby invading the mucosa. Using dominant-negative mutants, TW uptake was s

SUBMITTER: Friebel J 

PROVIDER: S-EPMC10094206 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature