Effect and Mechanism of Theaflavins on Fluoride Transport and Absorption in Caco-2 Cells.

ABSTRACT: This paper investigated the effect and mechanism of theaflavins (TFs) on fluoride (F^-) uptake and transport in the Caco-2 cell model through structural chemistry and transcriptome analysis. The results showed that the four major TFs (TF, TF3G, TF3'G and TFDG) at a 150 μg/mL concentration could all significantly decrease F^- transport in Caco-2 cells after 2 h of treatment and, at 2 μg/mL F^- concentration, the F^- transport was more inclined to efflux. During transport, the F^- retention in Caco-2 cells was significantly increased by TF3G while it was clearly decreased by TF. The interaction between TFs and F^- was analyzed by Raman spectroscopy and isothermal titration calorimetry, and F^- was shown to affect the π bon

SUBMITTER: Fan Y 

PROVIDER: S-EPMC10094491 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature