LncRNA <i>TCONS_00323213</i> Promotes Myogenic Differentiation by Interacting with PKNOX2 to Upregulate <i>MyoG</i> in Porcine Satellite Cells.

ABSTRACT: Myogenic differentiation is a complex biological process that is regulated by multiple factors, among which long noncoding RNAs (lncRNAs) play an essential role. However, in-depth studies on the regulatory mechanisms of long noncoding RNAs (lncRNAs) in myogenic differentiation are limited. In this study, we characterized the role of the novel lncRNA TCONS_00323213, which is upregulated during porcine skeletal muscle satellite cell (PSC) differentiation in myogenesis. We found that TCONS_00323213 affected the proliferation and differentiation of PSC in vitro. We performed quantitative polymerase chain reaction (qPCR), 5-ethynyl-20-deoxyuridine (EdU), western blotting, immunofluorescence staining, pull-down assays, and cleavage under targets and tagmentation (CUT and Tag) assay

SUBMITTER: Li M 

PROVIDER: S-EPMC10094759 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature