Analysis of RNA Polyadenylation in Healthy and Osteoarthritic Human Articular Cartilage.

ABSTRACT: Polyadenylation (polyA) defines the 3' boundary of a transcript's genetic information. Its position can vary and alternative polyadenylation (APA) transcripts can exist for a gene. This causes variance in 3' regulatory domains and can affect coding sequence if intronic events occur. The distribution of polyA sites on articular chondrocyte transcripts has not been studied so we aimed to define their transcriptome-wide location in age-matched healthy and osteoarthritic knee articular cartilage. Total RNA was isolated from frozen tissue samples and analysed using the QuantSeq-Reverse 3' RNA sequencing approach, where each read runs 3' to 5' from within the polyA tail into the transcript and contains a distinct polyA site. Differential expression of transcripts was significant altered between

SUBMITTER: Winstanley-Zarach P 

PROVIDER: S-EPMC10094766 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature