The Influence of Edaphic Factors on DNA Damage and Repair in Wild Wheat Triticum dicoccoides Korn. (Poaceae, Triticeae).

ABSTRACT: A complex DNA repair network maintains genome integrity and genetic stability. In this study, the influence of edaphic factors on DNA damage and repair in wild wheat Triticum dicoccoides was addressed. Plants inhabiting two abutting microsites with dry terra rossa and humid basalt soils were studied. The relative expression level of seven genes involved in DNA repair pathways-RAD51, BRCA1, LigIV, KU70, MLH1, MSH2, and MRE11-was assessed using quantitative real-time PCR (qPCR). Immunolocalization of RAD51, LigIV, γH2AX, RNA Polymerase II, and DNA-RNA hybrid [S9.6] (R-loops) in somatic interphase nuclei and metaphase chromosomes was carried out in parallel. The results showed a lower expression level of genes involved in DNA repair and

SUBMITTER: Raskina O 

PROVIDER: S-EPMC10094829 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature