Monitoring Nrf2/ARE Pathway Activity with a New Zebrafish Reporter System.

ABSTRACT: Among multiple cytoprotective mechanisms, eukaryotic cells exhibit a complex transcriptional program relying on the Nrf2 transcription factor, which is generally recruited upon biological stressors including oxidative-stress-based cellular insults. The relevance of this master regulator has remarkably emerged in recent years in several research fields such as cancer, inflammatory disorders and age-related neurological diseases. Here, we document the generation and characterization of a novel Nrf2/ARE pathway biosensor fish which exhibits a dynamic spatiotemporal expression profile during the early developmental stages. The transgenic line is responsive to known Nrf2 pathway modulators but also to Edaravone, which direct activity on the Nrf2 pathway has never been documented in a live trans

SUBMITTER: Badenetti L 

PROVIDER: S-EPMC10095123 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature