Inhibition of Soluble Epoxide Hydrolase Activity by Components of Glycyrrhiza uralensis.

ABSTRACT: Soluble epoxide hydrolase (sEH) is a target enzyme for the treatment of inflammation and cardiovascular disease. A Glycyrrhiza uralensis extract exhibited ~50% inhibition of sEH at 100 μg/mL, and column chromatography yielded compounds 1-11. Inhibitors 1, 4-6, 9, and 11 were non-competitive; inhibitors 3, 7, 8, and 10 were competitive. The IC₅₀ value of inhibitor 10 was below 2 μM. Molecular simulation was used to identify the sEH binding site. Glycycoumarin (10) requires further evaluation in cells and animals.

SUBMITTER: Kim JH 

PROVIDER: S-EPMC10095357 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature