Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Lignans from the Roots and Rhizomes of Dysosma versipellis and Their Cytotoxic Activities.

ABSTRACT: One new dibenzyltyrolactone lignan dysoslignan A (1), three new arylnaphthalide lignans dysoslignan B-C (2-4), along with fourteen known metabolites (5-18), were isolated from the roots and rhizomes of Dysosma versipellis. Their structures and stereochemistry were determined from analysis of NMR spectroscopic and circular dichroism (CD) data. Compound 2 represents the first report of naturally occurring arylnaphthalide lignan triglycoside. The cytotoxic activities of all isolated compounds were evaluated against A-549 and SMMC-7721 cell lines. Compounds 7-10 and 14-16 were more toxic than cisplatin in two tumor cell lines. This investigation clarifies the potential effective substance basis of D. versipellis in tumor treatment.

SUBMITTER: Sun Y

PROVIDER: S-EPMC10095701 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Lignans from the Roots and Rhizomes of Dysosma versipellis and Their Cytotoxic Activities.

Sun Yanjun Y Wang Haojie H Han Ruijie R Bai Hongyun H Li Meng M Wang Junmin J Feng Weisheng W

Molecules (Basel, Switzerland) 20230324 7

One new dibenzyltyrolactone lignan dysoslignan A (1), three new arylnaphthalide lignans dysoslignan B-C (2-4), along with fourteen known metabolites (5-18), were isolated from the roots and rhizomes of Dysosma versipellis. Their structures and stereochemistry were determined from analysis of NMR spectroscopic and circular dichroism (CD) data. Compound 2 represents the first report of naturally occurring arylnaphthalide lignan triglycoside. The cyt ...[more]

PMID: 37049673

Similar Datasets

Dysosma versipellis

Project description:Dysosma versipellis Genome sequencing and assembly

| PRJNA418373 | ENA

Dysosma versipellis

Project description:Dysosma versipellis linage 2 Genome sequencing and assembly

| PRJNA418383 | ENA

Dysosma versipellis

Project description:Dysosma versipellis overview

| PRJNA418622 | ENA

Dysosma versipellis

Project description:Dysosma versipellis Transcriptome or Gene expression

| PRJNA245785 | ENA

Alleviation of podophyllotoxin toxicity using coexisting flavonoids from Dysosma versipellis.

Project description:Podophyllotoxin (POD) is a lignan-type toxin existing in many herbs used in folk medicine. Until now, no effective strategy is available for the management of POD intoxication. This study aims to determine the protective effects of ?avonoids (quercetin and kaempferol) on POD-induced toxicity. In Vero cells, both ?avonoids protected POD-induced cytotoxicity by recovering alleviating G2/M arrest, decreasing ROS generation and changes of membrane potential, and recovering microtubule structure. In Swiss mice, the group given both POD and ?avonoids group had significantly lower mortality rate and showed less damages in the liver and kidney than the group given POD alone. As compared to the POD group, the POD plus ?avonoids group exhibited decreases in plasma transaminases, alkaline phosphatase, lactate dehydrogenase, plasma urea, creatinine and malondialdehyde levels, and increases in superoxide dismutase and glutathione levels. Histological examination of the liver and kidney showed less pathological changes in the treatment of POD plus ?avonoids group. The protective mechanisms were due to the antioxidant activity of ?avonoids against the oxidative stress induced by POD and the competitive binding of ?avonoids against POD for the same colchicines-binding sites. The latter binding was confirmed by the tubulin assembly assay in combination with molecular docking analyses. In conclusion, this study for the first time demonstrated that the coexisting flavonoids have great protective effects against the POD toxicity, and results of this study highlighted the great potential of searching for effective antidotes against toxins based on the pharmacological clues.

| S-EPMC3749096 | biostudies-literature

Whole-Genome Sequence of the Endophytic Actinacidiphila bryophytorum Strain DS3, Isolated from the Roots of the Medicinal Plant Dysosma versipellis.

Project description:We report the whole-genome sequence of the endophytic Actinacidiphila bryophytorum strain DS3, which was isolated from the roots of the medicinal plant Dysosma versipellis. The DS3 strain genome consists of a chromosome of 8,265,668 bp, with a GC content of 72.47%, including 7,121 coding sequences.

| S-EPMC10019196 | biostudies-literature

Structurally diverse biflavonoids from Dysosma versipellis and their bioactivity.

Project description:Five pairs of new biflavonoid enantiomers, (±)-dysosmabiflavonoids A-E (1-5), two new biflavonoids, dysosmabiflavonoids F-G (6-7), and four biosynthetically related precursors (8-11) were isolated from the roots and rhizomes of Dysosma versipellis. Their structures were elucidated by extensive spectroscopic analysis, including HR-ESI-MS and 2D NMR. Their absolute configurations were determined by comparison of the calculated and experimental ECD spectra. All isolated compounds were evaluated for AChE inhibitory activity. Compounds 6 and 7 exhibited more potent inhibitory activities with IC50 values of 1.42 and 0.73 µM, respectively, than their biosynthetically related precursors kaempferol (8, 17.90 µM) and quercetin (9, 3.96 µM). The preliminary structure-activity relationship study indicated that the connection mode of biflavonoid subunits, oxidation degree of the C ring, and 3,4-dihydroxy group of the B ring were important structural factors for AChE inhibitory activity. Racemates 1-5 and their corresponding levorotatory and dextrorotatory enantiomers were tested for their potential to impede the generation of NO in lipopolysaccharide-stimulated RAW264.7 cells, and their mushroom tyrosinase inhibitory effect. Racemate 1 displayed more potent mushroom tyrosinase inhibitory activity (IC50, 28.27 µM) than the positive control kojic acid (IC50, 32.59 µM). D. versipellis may have therapeutic potential for melanogenesis disorders and neurodegenerative diseases.

| S-EPMC9728092 | biostudies-literature

Diversity and bioactive potential of culturable fungal endophytes of Dysosma versipellis; a rare medicinal plant endemic to China.

Project description:The plant Dysosma versipellis is known for its antimicrobial and anticancer properties but is a rare and vulnerable perennial herb that is endemic to China. In this study, 224 isolates were isolated from various tissues of D. versipellis, and were classified into 53 different morphotypes according to culture characteristics and were identified by sequence analyses of the internal transcribed spacer (ITS) region of the rRNA gene. Although nine strains were not assignable at the phylum level, 44 belonged to at least 29 genera of 15 orders of Ascomycota (93%), Basidiomycota (6%), and Zygomycota (1%). Subsequent assays revealed antimicrobial activities of 19% of endophytic extracts against at least one pathogenic bacterium or fungus. Antimicrobial activity was also determined using the agar diffusion method and was most prominent in extracts from four isolates. Moreover, high performance liquid chromatography (HPLC) and ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry analyses (UPLC-QTOF MS) showed the presence of podophyllotoxin in two Fusarium strains, with the highest yield of 277 μg/g in Fusarium sp. (WB5121). Taken together, the present data suggest that various endophytic fungi of D. versipellis could be exploited as sources of novel natural antimicrobial or anticancer agents.

| S-EPMC5897559 | biostudies-literature

Complete chloroplast genome sequence of Dysosma versipellis (Berberidaceae), a rare and threatened species endemic to China.

Project description:Dysosma versipellis (Berberidaceae) is a rare and threatened medicinal herb endemic to subtropical China. Here, we first report and characterize its complete chloroplast genome based on Illumina paired-end sequencing data. The complete plastid genome was 156,735 bp, which contained inverted repeats (IR) of 25,925 bp separated by a large single copy (LSC) and a small single copy (SSC) of 86,514 bp and 18,371 bp, respectively. The cpDNA contains 133 genes, comprising 86 protein-coding genes, 37 tRNA genes, 8 rRNA genes, and 2 processed pseudogenes. The overall GC content of the plastome is 38.5%. The phylogenetic analysis of 17 selected chloroplast genomes demonstrated that D. versipellis is closely related to the congeneric D. pleiantha.

| S-EPMC7707710 | biostudies-literature

Kaempferol Identified by Zebrafish Assay and Fine Fractionations Strategy from Dysosma versipellis Inhibits Angiogenesis through VEGF and FGF Pathways.

Project description:Natural products are a rich resource for the discovery of therapeutic substances. By directly using 504 fine fractions from isolated traditional Chinese medicine plants, we performed a transgenic zebrafish based screen for anti-angiogenesis substances. One fraction, DYVE-D3, was found to inhibit the growth of intersegmental vessels in the zebrafish vasculature. Bioassay-guided isolation of DYVE-D3 indicates that the flavonoid kaempferol was the active substance. Kaempferol also inhibited the proliferation and migration of HUVECs in vitro. Furthermore, we found that kaempferol suppressed angiogenesis through inhibiting VEGFR2 expression, which can be enhanced by FGF inhibition. In summary, this study shows that the construction of fine fraction libraries allows efficient identification of active substances from natural products.

| S-EPMC4597183 | biostudies-literature