Target pollen isolation using automated infrared laser-mediated cell disruption.

ABSTRACT: Single-cell analysis is important to understand how individual cells work and respond at the cell population level. Experimental single-cell isolation techniques, including dilution, fluorescence-activated cell sorting, microfluidics, and micromanipulation, have been developed in recent decades. However, such applications typically require large cell populations and skilled professionals. Additionally, these methods are unsuitable for sequential analysis before and after cell isolation. In this study, we propose a method for target cell isolation using automated infrared laser-mediated disruption of pollen grains in pollen populations. Germination of the target pollen was observed at the same location as that before laser irradiation, and germinated pollen grains were enriched in the cell

SUBMITTER: Kaneshiro I 

PROVIDER: S-EPMC10095853 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature