Spatio-temporal imaging of cell fate dynamics in single plant cells using luminescence microscope.

ABSTRACT: Stem cell fates are spatio-temporally regulated during plant development. Time-lapse imaging of fluorescence reporters is the most widely used method for spatio-temporal analysis of biological processes. However, excitation light for imaging fluorescence reporters causes autofluorescence and photobleaching. Unlike fluorescence reporters, luminescence proteins do not require excitation light, and therefore offer an alternative reporter for long-term and quantitative spatio-temporal analysis. We established an imaging system for luciferase, which enabled monitoring cell fate marker dynamics during vascular development in a vascular cell induction system called VISUAL. Single cells expressing the cambium marker, proAtHB8:ELUC, had sharp luminescence peaks at different time points. Furt

SUBMITTER: Shimadzu S 

PROVIDER: S-EPMC10095866 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature