In-Depth Performance Analysis and Comparison of Monolithic and Particulate Zwitterionic Hydrophilic Interaction Liquid Chromatography Polymer Columns.

ABSTRACT: The kinetic performance of different zwitterionic hydrophilic interaction liquid chromatography polymer columns is evaluated and compared in-depth. For this purpose, two lab-made monolithic columns, synthesized with different crosslinkers, and a commercial particle packed column are considered. It is found that performance evaluation techniques, such as comparing plate height curves or fitted A-, B- and C-terms, obtained by fitting experimental plate height data to a plate height model, are complicated by the determination of a reliable characteristic length. This is due to the very different morphology of these column types, and the heterogeneity of the monolithic columns. The occurrence of a convective flow through the packed particle column further complicates the interpretation of the

SUBMITTER: Li H 

PROVIDER: S-EPMC10095884 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature