Purification, Identification and Evaluation of Antioxidant Peptides from Pea Protein Hydrolysates.

ABSTRACT: Food-derived antioxidant peptides can be explored as natural antioxidants due to their potential health benefits. In this study, antioxidant peptides were isolated and purified from pea protein hydrolysates (PPH). The DPPH and ABTS radical scavenging activities were used as indexes to purify the antioxidant peptides by a series of purification steps including ultrafiltration, ion exchange chromatography, G25 gel filtration chromatography, and reversed-phase chromatography. Three novel antioxidant peptides YLVN, EEHLCFR and TFY were identified, which all exhibited strong antioxidant activity in vitro. EEHLCFR showed stronger DPPH scavenging activity with an IC₅₀ value of 0.027 mg/mL. YLVN showed stronger ABTS scavenging activity with an IC₅₀ value of 0.002 mg/mL and hi

SUBMITTER: Zhao D 

PROVIDER: S-EPMC10096042 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature