Combined action of albumin and heparin regulates lipoprotein lipase oligomerization, stability, and ligand interactions.

ABSTRACT: Lipoprotein lipase (LPL), a crucial enzyme in the intravascular hydrolysis of triglyceride-rich lipoproteins, is a potential drug target for the treatment of hypertriglyceridemia. The activity and stability of LPL are influenced by a complex ligand network. Previous studies performed in dilute solutions suggest that LPL can appear in various oligomeric states. However, it was not known how the physiological environment, that is blood plasma, affects the action of LPL. In the current study, we demonstrate that albumin, the major protein component in blood plasma, has a significant impact on LPL stability, oligomerization, and ligand interactions. The effects induced by albumin could not solely be reproduced by the macromolecular crowding effect. Stabilization, isothermal titration calorimet

SUBMITTER: Risti R 

PROVIDER: S-EPMC10096250 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature